Predoctoral Trainee

Dalia Dhingra Chemistry Doctoral Training Program Dept. of Chemistry Faculty Supervisor: David C. Schwartz Email: dhingra@lmcg.wisc.edu 265-7946

The research group that I am affiliated with develops systems for genomic analysis based on the elucidation and integration of single molecule effects. One such system created in the group, referred to as Optical Mapping, utilizes fluorescence microscopy to directly analyze DNA molecules bound to chemically altered surfaces. An even more powerful single molecule system being developed in the laboratory is aimed at acquiring information from ensembles fixed DNA molecules, and is aptly named – Optical Sequencing. My research is directed towards the advancement of the Optical Sequencing system to the point where it is sufficiently robust to produce large datasets. Although many aspects of this system are already in place, there are several important issues to be addressed, which will require concerted efforts spanning across several disciplines. More specifically, the primary measurements hinge on the stable assessment of errors associated with single fluorochrome quantitation. I will be developing error models to account for spurious polymerase mediated additions, multiple fluorochromes, and the indexing of such biochemical actions with large DNA templates. The final goal is to fully automate the sequence acquisition steps and analysis and to link such datasets with public sequence databases. Such linkage will enable the discernment of mutations that stem from SNP’s, or other chromosomal aberrations.

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File last modified: August 13, 2003
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