Predoctoral Trainee

Jason K. Middleton Chemical Engineering Doctoral Training Program Dept. of Chemical Engineering Faculty Supervisor: John Yin Email: jkmiddleton@students.wisc.edu 265-3781

Heat inactivation of two reovirus particles, the virion and the intermediate sub-virion particle (SVP) using two reovirus strains type 1 Lang (T1L) and type 3 Dearing (T3D). We determined that the phenotype difference in ISVP inactivation of these two strains maps to the M2 gene using viral reassortants. In addition, the ISVPs exhibit first-order kinetics and our kinetic analysis produces a linear Arrhenius plot. We also demonstrate that viral half life is a good parameter for studying the genetic determinants of the phenotype difference. In conclusion, we show that virions are stable to higher temperature than ISVPs and exhibit non-first-order inactivation kinetics.

Resume

Resume 2002 (.pdf format, Download Reader)

Publications

Middleton JK (speaker) and Yin J. Intracellular Auto-amplification of Viral Genomes. Presented at the AIChE 2002 National Meeting, Indianapolis, IN.

Middleton JK, Severson TF, Chandran K, Gillian AL, Yin J, and Nibert ML. Thermostability of reovirus disassembly intermediates (ISVPs) correlates with genetic, biochemical, and thermodynamic properties of major surface protein mu1. J. Virol. 76: 1051-1061, 2002.

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